In vitro Plant Regeneration of Two Cucumis melo L. Genotypes Using Different Explant Types and Culture Medium

Stanislava Y. Grozeva*, Nikolay V. Velkov, Zhana V. Ivanova
 
Maritsa Vegetable Crops Research Institute – Plovdiv, 32 Bresovsko shosse Str., 4003 Plovdiv, BULGARIA
*Corresponding author: stanislava_grozeva@abv.bg
 
Abstract. Development of an efficient in vitro plant regeneration system plays an important role for applying of biotechnological approaches for crop improvement. Therefore, the effect of added of BAP in germination phase on organogenesis of cotyledon and hypocotyl explants of two melon lines was investigated. The seeds germination medium contained three different concentrations of BAP (0.5, 1.0 and 1.5 mg L-1). The rate of plant regeneration was found to depend on genotype, explant type and culture medium. The cotyledons were more effective as explants for organogenesis and subsequent plant elongation than hypocotyls. Combination of 1.0 mg L-1 of BAP in germination medium and 0.5 mg L-1 BAP + 0.5 mg L-1 IAA in next regeneration medium gives the better regeneration answer in two explant types of line 11/9, while in line AGY the most effective was the combination of 1.5 mg L-1 of BAP and 0.5 mg L-1 BAP + 0.5 mg L-1 IAA. The experimental results indicated that pre-treatment with cytokinin BAP (1.0 mg L-1) in germination stage stimulates regeneration process in cotyledons and hypocotyls of melon line 11/9.
 
Key words: melon, cotyledons, hypocotyls, pre-treatment, cytokinin.
 

Ecologia Balkanica, 2019, vol. 11, Issue 2, pp. 193-202
Article № eb.19147 [Full text - PDF]pdf

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