Comparison of Endophytic Colonization of Bulgarian Variety of Tobacco by Enthomopathogenic Fungi - Beauveria bassiana and Beauveria brongniartii

Mariana K. Petkova1*, Velichka V. Spasova-Apostolova2, Veselina B. Masheva3, Nurettin T. Tahsin4
 
1 - Agricultural University, Faculty of Plant Protection and Agroecology, Department of Microbiology and Environmental Biotechnology, Plovdiv 4000, BULGARIA
2 - Tobacco and Tobacco Products Institute, Department of Breeding and Seed Production, Markovo 4108, BULGARIA
3 - Institute of Plant Genetic Resources "Konstantin Malkov", Department of Plant Genetic Resources, Sadovo 4122, BULGARIA
4 - Agricultural University, Faculty of Agronomy, Department of Crop Science, Plovdiv 4000, BULGARIA
*Corresponding author: marandonova@gmail.com
 
Abstract. In the modern breeding programs, the application and utilization of endophytic potential of microorganism is an opportunity to reduce damage from different pests and viruses on tobacco plants. In the present study, 48 plants of 56 day seedlings of oriental tobacco (Krumovgrad 58) plants treated with 2 strains 538 and 730 of the entomopathogenic fungi Beauveria bassiana and a strain 646 of Beauveria brongniartii. Two different inoculation techniques were applied by spraying the leaves and by directly placing the inoculant in the soil near the root of the plants. In order to compare the effectiveness of the colonization techniques of different tobacco tissues roots, stems and leaves, samples were taken for analysis on 7, 21 and 28 days after inoculation. Results have proven that all three strains of Beauveria endophyticaly colonize different tobacco tissues within 28 days after inoculation. The outcomes of the present study show the potential of B. bassiana and B. brongniartii to use for prevention and protection of tobacco plants.
 
Key words: Beauveria bassiana, Beauveria brongniartii, Nicotiana tabaccum, endophyte.
 

Ecologia Balkanica, 2020, Volume 12, Issue 1 pp. 31-40
Article № eb.20115 [Full text - PDF]pdf

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